ATTO 647

ATTO 647 is a carborhodamine fluorophore and imaging tracer with photostable properties. ATTO 647 serves as a fluorescent probe to investigate cell membrane structure and diffusion characteristics. When conjugated with wheat germ agglutinin, ATTO 647 specifically binds to N-acetyl-β-D-glucosamine and sialic acid residues on membrane glycoproteins, enabling single-molecule tracing of glycoprotein diffusion. ATTO 647 exhibits highly stable fluorescence properties with significantly reduced blinking in mounting media such as ROXS (AA/MV) and ROXS (TX/TQ), whereas its brightness properties vary in Ibidi-MM and Vectashield. ATTO 647 can also be used to label histone H2B-GFP in fixed cells for confocal microscopy photobleaching experiments.


Cat. No.: EX-A13456	Purity: >98%

	Chemical structure of ATTO 647

For research only, Do not use for Human!

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CAS No.	906664-68-4
Purity	>98%
Formula	C34H45ClN2O9S
Mol Weight	693.247
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.
Shipping	Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Reference:	[1]. Bruckbauer A, et al. Nanopipette delivery of individual molecules to cellular compartments for single-molecule fluorescence tracking. Biophys J. 2007;93(9):3120-3131. [2]. Cordes T, et al. Mechanisms and advancement of antifading agents for fluorescence microscopy and single-molecule spectroscopy. Phys Chem Chem Phys. 2011;13(14):6699-6709. [3]. Kocabey S, et al. Cellular Uptake of Tile-Assembled DNA Nanotubes. Nanomaterials (Basel). 2014;5(1):47-60. Published 2014 Dec 30.
CoA	CoA of ATTO 647

Smiles:	O=C(O)CCCN1C=2C=C3C(C=C4C=C5C(C=C4C3(C)C)=[N+](CC)C(C=C5CS(=O)(=O)O)(C)C)=CC2C(C)CC1(C)C.O=Cl(=O)(=O)[O-]


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